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Antoine Valera
PhD student in Neurosciences
EDUCATION
Ph.D. student in Neurosciences - University of Strasbourg, France : Thesis : Modular organisation in the cerebellum
Master in Neuroscience : Cellular and Integrative Neurosciences - University of Strasbourg, France : Master's thesis : High frequency transmission at the Parallel fiber to Purkinje Cell synapse.
Bachelor in Science : Cell Biology and Physiology - University of Strasbourg, France
PUBLICATIONS & POSTER COMMUNICATIONS
Publications
Valera et al., J. Neurosci., 2012,
Adaptation of Granule Cell to Purkinje Cell Synapses to High-Frequency Transmission.
Lonchamp E, Gambino F, Dupont JL, Doussau F, Valera A, Poulain B, Bossu JL, Plos One, 2012
Pre and Post Synaptic NMDA Effects Targeting Purkinje Cells in the Mouse Cerebellar Cortex.
Kaufling J, Waltisperger E, Bourdy R, Valera A, Veinante P, Freund-Mercier MJ, Barrot M., Br. J. Pharmacol. 2010
Pharmacological recruitment of the GABAergic tail of the ventral tegmental area by acute drug exposure.
Posters
SFN annual meeting 2012, New Orleans.
Valera A. , Dupont J.-L. , Cruveiller H. , Léna C. , Pawlowski S. A. , Binda F. , Rothstein J. , Poulain B. & Isope P.
Functional Organization of Synaptic Inputs on Mouse Cerebellar Golgi Cells Related to Zebrin Band Patterns
8th FENS, Barcelona 2012.
Valera A. , Dupont J.-L. , Cruveiller H. , Léna C. , Pawlowski S. A. , Binda F. , Rothstein J. , Poulain B. & Isope P.
Functional Organization of Synaptic Inputs on Mouse Cerebellar Golgi Cells Related to Zebrin Band Patterns
Doussau F., Valera A., Poulain B. & Isope P
Low-Frequency Depression at the cerebellar granule cell to Purkinje cell synapse
Chaumont J., Selimi F., Guyon N., Gautheron G., Reibel-Foisset S., Popa D., Valera A., Dieudonné S., Barrot M., Cassel J-C., Poulain B., Doussau F., Léna C. and Isope P.
Modulation of cerebellar spatio-temporal activity by optogenetic stimulation of channelrhodopsin specifically expressed in Purkinje cells
10th Neurosciences colloquium, Marseille 2011.
Valera, A. , Barbour, B., Poulain, B., Isope, P.
Characterization of short term plasticity during high frequency bursts at parallel fiber - Purkinje cells synapse
7th FENS, Amsterdam 2010.
Valera A. , Barbour B. , Poulain B. & Isope P.
Characterization of short term plasticity during high frequency bursts at parallel fiber – Purkinje cells synapse.
Kneib M., Gambino F. , Valera A. , Cruveiller H. , Rothstein J. , Isope P. & Humeau Y.
Segmentation of inhibition in the cerebellar cortex
INTERNSHIPS
I.N.C.I, Neuroendocrine secretion and Neurotransmission Department, Strasbourg.
Supervisor : Philippe Isope; High Frequency Transmission at Parrallel Fiber-->Purkinje Cell Synapse.
-Acute Slices, Electrophysiology, Igor Pro programming and analysis, SigmaPlot analysis.
I.N.C.I, Neuroendocrine secretion and Neurotransmission Department, Strasbourg.
Supervisor : Yann Humeau ; Electrophysiological characterization of inhibitory ML interneurons in the cerebellar cortex.
-Electrophysiology, PClamp Analysis.bellar cortex.
I.N.C.I, Pain and Nociception Department, Strasbourg.
Supervisor : Michel Barrot ; c-fos expression in the GABAergic tail of the ventral tegmental area.
-Immunohistochemestry.
I.B.M.C, Genetic of Inflammatory Response, Strasbourg
Supervisor : Vincent Leclerc. Innate immunity in Drosophilia.
-Western blot, genetic Drosophilia selection.
OTHER RESEARCH EXPERIENCES
Teaching:
Teaching assistant in Neuroanatomy, Neurophysiology and Cell Biology
Animal experiment agreement & surgery training.
ENP summer school in Paris:
Overview of advanced electrophysiological and optical tool. Practical work (dendritic patch, in vivo recordings)
Electrophysiology summer school in Strasbourg:
Overview of various electrophysiological tools, Electrophysiology fundamental concepts.
SKILLS
Whole cell patch clamp (including double patch clamp), cell attached recordings on acute slices.
Confocal microscopy, 3D reconstruction. Occasional 2 photon and calcium imaging
Variance-mean analysis (see Valera et al., J Neurosci., 2012).
Cerebellar cortex network mapping using RuBi Glutamate uncaging (currently used technique)
All my analyses are performed using homemade programs :
Python and MySQL Programming. Homemade Software for data exploration and analyses using databases (SynaptiQs).
Igor Pro Homemade procedures for analysis (used in Valera et al., J Neurosci., 2012)
Languages
French : Native language
English : Fluent
German and Italian : Working knowledge
Computer Skills
Signal Analysis:
IgorPro programming
Python / PyQT programming
SQL programming notions
SigmaPlot, for statistics.
Image Analysis:
ImageJ
Adobe Photoshop
Adobe Illustrator
